We are now on week 10 of the current course, covering The Oxidative Stress test. This is also known as Dry Blood Analysis where a slide is applied to a pinprick of blood 8 times to get 8 layers on the slide. The sample is left to dry and then viewed under the microscope.

White areas that are often seen in the dry blood sample (as above) that look like holes in the sample are soft clots called polymerized protein puddles (PPPs) these are associated with free radical damage/oxidative stress and toxins.

PPPs may vary in size, ranging from small white dots to larger lakes or rivers. They indicate the degree of tissue health and are associated with free radical damage/oxidative stress and toxins.

The shape and size of PPPs indicate the nature of the condition.

Round PPPs that are clear on the inside indicate irritations and inflammation (usually accompanied by pain in the area affected) whereas large, jagged PPPs with crystals indicate degenerative conditions.

PPPs of approximately 2 microns (pinpoints) are related to hypersensitivities and allergic conditions. These usually appear as a “snowstorm” and often throughout the sample.

PPPs of 30 microns are seen in association with physical, mental and emotional stress. These occur primarily in the centre of the sample.

PPPs of 40 microns and larger are indicative of degenerative conditions (when they are not round and clear).

The larger the PPPs and the greater the number the more serious the condition.

When PPPs are observed in deeper layers it indicates chronic conditions.

When PPPs appear localized in a specific area in the sample (not throughout) it indicates localization of the problem in the body, rather than systemic conditions.

When large PPPs are observed throughout the sample in all the areas, it indicates toxicity and advanced degenerative conditions.

In The Oxidative Stress Test (Dry Blood Analysis)  a lot can be learned by the size, the shape and the position of the PPP’s.

Uric Acid Crystals

These appear as crystals that look like broken glass, yellow-green (brightfield) and highly reflective (darkfield).

These occur as a result of poor digestion of dietary protein and/or poor elimination of uric acid. The system is acidic and some degree of inflammation may be present.

Improving protein digestion is very important. Digestive enzymes are usually used in addition to limiting meat & chicken to 1g/kg of body weight. Alkaline-forming supplements (base powders, green extracts) and an 80% alkaline-forming and 20% acid-forming diet is recommended, along with other nutritional interventions.


Uric acid crystals appear as light yellow to green crystals in brightfield and bright, highly reflective crystals in darkfield that look like broken glass.


Uric acid crystals are one of the most commonly observed crystals in live blood samples. More than 2 or 3 small crystals in more than 5 fields (under 400x magnification) should be considered significant, or 2 or 3 large crystals (as pictured above) in one sample.


Uric acid crystals can be seen in live blood samples of patients who also display protein linkage and/or kidney stress. Considered by some to be dried protein accumulations rather than uric acid itself, these crystals suggest a problem with protein digestion, over-acidity and/or elimination by the kidneys. Always ask about water intake.

Associated Symptoms:

♦ Joint pain and stiffness. This may range from mild tenderness and stiffness (typically worse after rest and on waking) to severe acute pain.

♦ Slow recovery after exercise, prolonged stiffness.


♦ Digestive Enzymes

♦ Limit protein

♦ Base powder and alkaline-forming diet.

♦ Herbal Kidney / Uric acid support, including celery seed, devil’s claw, etc.

♦ Raw vegetable juices, especially celery juice.

♦ Increase water intake. To determine necessary daily water intake (in litres): Weight (kg) ÷ 8) x 0.25.

♦ Reduce intake of purine-rich foods (e.g.: liver, kidney, heart, game meats, meat extracts, anchovies, scallops, mussels, mackerel and gravy).

♦ Reduce intake of dietary animal protein to 1g per kg body weight per day (e.g.: a 70kg adult should not exceed 70g animal protein daily).

♦ Do not consume animal protein and simple starch in the same meal.

♦ Eat meals while sitting down, do not rush while eating – remember to chew properly.

♦ Follow the diet relevant to the blood type.

Cholesterol Crystals Seen In Live Blood Analysis

Many different types of crystal formations can be observed in live blood samples and vary greatly in size, shape and colour.


They always appear as solid objects that are usually much larger than red blood cells.

Crystal formation in the blood is a sclerotic process that is related to inflammation. The presence of crystalline forms is often associated with high blood pressure, plaque and atherosclerosis. There are often white blood cells attached and attracted to crystal formations in the blood, attempting to engulf the crystals.

This is a favourable sign and an indication of an active immune system




In Live Blood Analysis we view the clients live blood magnified onto a screen.

We look at the Red Blood Cells (RBCs), the white blood cells (WBC’s), the platelets and the plasma.

We compare the client’s blood picture to normal healthy blood and look for anomalies in the size & shape of the cells, if they are free floating or sticking together, too many, too few, bacterial forms, yeast markers, fibrin spicules, crystals or protoplasts in the plasma.


“Cholesterol Crystals in the blood” is taken from the 500+ page Live Blood Online Training Manual



Cholesterol crystals are trapezoidal structures of varying sizes that appear semi-opaque in

brightfield and semi-reflective in darkfield.



These trapezoidal crystals are usually associated with atherosclerotic plaque and considered

significant if observed during analysis. Their elimination may be stimulated by specific natural

treatment protocols.



♦ Indication of possible high serum cholesterol.

♦ Possible excessive saturated fat intake.

♦ Inadequate lipid metabolism due to lipase deficiency.

♦ Atherosclerotic plaque.


Associated Symptoms:

♦ Fatigue

♦ May be asymptomatic (no symptoms)

♦ Poor circulation

♦ Angina

For more on:

Pleomorphic Perspective

Medical Perspective


Further Investigations:

Please join our online training course September 26th 2017

Natural Health Treatments

Natural Heath Treatments – Are they working?

Many practitioners feel like they’re working in the dark when treating chronic cases. This is because chronic cases typically take a long time to respond to treatment, and patients don’t notice an improvement in the first few months of treatment. Most successful practitioners rely on years of experience to know that their treatment is working. However, even these practitioners struggle with poor patient compliance, because if patients don’t feel better they lose faith and start looking for other alternatives.

  • How do you determine what the treatment is doing?
  • Is your treatment addressing the most important, fundamental issue in the patient’s case?
  • Have all the contributing factors and variables in the case been considered?
  • How are the patient’s diet and lifestyle influencing the effectiveness of your treatment?

These are some of the questions that natural health practitioners struggle with every day. Well, those natural health practitioners who are not using live blood analysis.

Live blood analysis can clearly show if treatment is progressing in the right direction. Changes seen in the patient’s sample will indicate whether the case is improving, and the abnormalities seen in the patient’s blood samples will highlight the most important areas to focus on. What’s more is because the patient can see for themselves that their condition is improving, they remain motivated and compliant during the whole treatment process, ensuring positive results in every case.

This is an example of a typical live and dry blood sample before and after treatment. The blood samples are clearly improving, indicating that the patient’s health is improving at a cellular level.



For more information on how to make this powerful technique a part of your practice, please feel free to contact us

What Your White Blood Cells Actually Do

Watch What Your White Blood Cells Actually Do

We are on week 5 of the latest live blood analysis online training course and studying white blood cell (WBC’s) and WBC anomalies.

WBC’s in general relate to the state of the immune system, possible infections, allergies and a few other conditions, such as autoimmune diseases.

The video above is a speeded up video of a WBC chasing and eventually swallowing up a bacterial form – ingesting it by phagocytosis. WBC’s are also known as Leukocytes, they originate in the thymus, bone marrow and lymph tissue.

They are immune cells actively engaged in the destruction or neutralization of invading microorganisms. They are transported to sites of infection and inflammation. For this reason their lifespan in the blood in usually very short (around 14 days).

When infection is present they increase in number and they also become more mobile and move back and forth between the blood, lymph and the tissues. Neutrophil viability is one of the most important assessments used to determine the state of the immune system. The main criteria used for determining the viability of a neutrophil include size, condition and activity.

A neutrophil should be approximately twice as big as a RBC (approximately 14 microns in diameter). The main determinants in assessing the condition of a neutrophil are the condition of the cell’s border and segmentation. The border should be fairly smooth and regular and the neutrophil should not be hypersegmented nor macrocytic (oversized).

The most important factor to observe when assessing neutrophil viability is activity. Here we look at the granules within the cell’s cytoplasm. Ideally, there should be many of them actively streaming within the cell. The cell itself should also be stretching out its membrane in irregular shapes to move around actively in the plasma. Typically, neutrophil viability should be at least 75%.

Non-viable neutrophils are often round, symmetrical and immobile.   


Poor neutrophil viability may be caused by many factors and is indicative of an underactive immune system.

This may be caused by mineral deficiencies, ongoing infections, chronic fatigue, smoking, alcohol and sugar intake and digestive weakness.  Poor oxygenation, stress, lack of exercise, poor sleeping patterns and yeast overgrowth can also contribute.  


Immune supporting herbals and nutritionals (e.g.: Olive leaf, Echinacea, Cats Claw, Goldenseal, Calcium ascorbate 2g 2xdaily, Zinc, N-Acetyl-Cysteine, proanthocyanidins).

Probiotic supplement. Trace minerals.

Improving the patient’s lifestyle, dietary habits and overall health is important here to ensure the correction of the terrain. 

Copyright Dr Okker R. Botha, Johannesburg, South Africa, 2009


Zeta Potential – Getting The Right Balance

Anyone who begins a serious study of biological terrain will encounter the concept of zeta potential because it is a basic principle of the electrical properties of life itself.

In one sense the body is electric–or electrostatic.

In various industries, the concept of zeta potential is common knowledge. Zeta potential plays a critical role in many industrial processes. The manufacture of soap is one example. Water by itself does not always clean as well as it could. Sometimes the water needs to be made wetter. How can you have wetter water that becomes a better cleaner and disperser of dirt on dishes? By adding anionic surfactants to the water thereby changing its charge. The anionic soapy water does a better job of getting between the cationic dirt particles of the dirty dishes and disperses the garbage.

The area of paints and pigments is another example. Whether a quantity of pigment added to a base paint will coagulate and form a speckled mess or disperse into trillions of tiny particles each remaining separate and discrete thereby leaving an even colour, depends almost entirely on the electrical properties of the system.

In the industrial process of purifying water in treatment plants, zeta potential plays a crucial role.  In order to get out pollutants, the treatment facility pours in a highly cationic substances like aluminium sulphate which attracts the garbage to itself thereby coagulating or flocculating out the precipate. This floc becomes heavy and drops to the bottom of the holding tank thereby cleansing the water. (Note that if they miscalculate how much cationic aluminium to add to the water, some of that will stay in the water supply that arrives at your tap and this aluminized tap water is definitely not good for health as it coagulates elements of your own body fluids.)

In a general way of thinking which is overly simplistic, think of anions as dispersers, and cations as coagulators. Anions disperse things, cations bring things together. Further, you could say anionic leans alkaline, cationic leans acid.

The Molecular Reality.

Molecular compounds are composed of various atoms with electrons spinning in their orbits and is a mix of anionic and cationic components. The ratios of these anions to cations give indications as to the valence of the molecule or electrolyte. The ions of both anionic and cationic electrolytes may carry from one to four charges and are accordingly designated mono-, di-, tri-, or polyvalent type electrolytes.

When the electrolytes are negatively charged(anionic) they are written as 1:1, 1:2, 1:3, 1:4 to indicate their ratios and their respective ionic strength. The higher the ratio the more ionic strength to increase zeta potential and have a dispersionary effect.

The right ionic balance is good for humans.

When the electrolytes are positively charged (cationic) they are written as 1:1, 2:1, 3:1, 4:1. The higher these ratios, the more ionic strength to decrease zeta potential and coagulate, agglutinate, flocculate, sludge and downright clog up systems.

The wrong ionic balance is bad for humans.

Negative Charge – 1:1, 1:2, 1:3, 1:4. Ratios indicate ionic strength. Higher = more strength to increase zeta potential. Good for humans.

Positive Charge – 1:1, 2:1, 3:1, 4:1. Higher ratios here means more strength to decrease zeta potential. Bad for humans.

A lot of the processed foods with chemical preservatives, pesticide residue and additives are of a cationic 1:1, 2:1 nature.  Bad for humans. These foods have a natural zeta potential lowering effect on the blood.

Chlorine is a well-known cationic electrolyte and when viewed in light of the above material it is easy to understand why drinking chlorinated water can elevate the risk of cardiovascular problems.

As it is, blood is naturally maintained in a dispersed state that is just on the verge of beginning to sludge. This is required for an effective blood clotting mechanism so if you cut yourself you don’t bleed to death. The blood clotting mechanism is associated with the release and activation of prothrombin-thrombin which is a cationic polyelectrolyte.

Heparin on the other hand is an anionic polyvalent electrolyte dispersing agent and is used medically to relieve intravascular coagulation.

Now with blood at a natural precipice just ready to sludge, if we add negative health items to our diet that have a further sludging effect on our blood, the situation for health begins to deteriorate.