Dry Blood Analysis – week 9 on the training course

Dry Blood Analysis – week 9 on the training course

We are continuing with the dry blood analysis anomalies this week but under a higher magnification.

There are quite a few anomalies that can be seen under higher magnification that relate to the degree of imbalance of the terrain.

Under higher magnification in the dry blood analysis is where we see signs of parasites, heavy metal toxicity, viral spiking – associated with chronic viral activity along with many other   indications of oxidation and degeneration.

Signs of liver flukes are seen in this image, this is quite common and usually seen with signs of liver stress in the live blood analysis.

Information is provided on the training course on how to identify this anomaly and the appropriate herbal protocol and lifestyle change to clear them. This could take many months but the results can be seen on further analysis.

Join us on the next training course in this fascinating subject Tue April 2nd

Are There Naturally Occurring Pleomorphic Bacteria in the Blood of Healthy Humans?

Ascits in darkfield
Ascits in darkfield

Dark-field microscopy of blood from healthy individuals revealed the existence of pleomorphic microorganisms.

These bacteria exhibited limited growth and susceptibility to antibiotics and could be detected by fluorescent in situ hybridization and flow cytometry. They were further characterized by analysis of their 16S rRNA and gyrB genes.

In our search for spirochetes involved in Alzheimer’s disease (13), we observed pleomorphic bacteria in the blood of healthy human subjects by dark-field microscopy.

This was a surprising finding since it is generally acknowledged that the bloodstream in healthy humans is a sterile environment (7) except when there is a breach in the integrity of the tissue membranes (6).

However, the concept of the occurrence of bacteria in the blood of healthy humans is now more plausible because of cultivation-independent laboratory approaches.

The main techniques employed in such studies include PCR amplification and sequencing of the16S ribosomal DNA (rDNA). These methods have revealed the presence of a wide diversity of microorganisms in the environment, and indeed within the human body (12).

In this report we present evidence based on molecular phylogenetic techniques and light and electron microscopy, as well as other conventional microbiological methods, for the existence of a population of bacteria in healthy human blood.

In view of the apparent controversial nature of our findings, it was encouraging to note the recent report of Nikkari et al. (14), who detected blood-associated bacterial rDNA sequences by using real-time PCR methods and a probe targeting conserved regions of bacterial 16S rDNA, and an earlier report by Tedeshi et al. (16) on the presence of pleomorphic bacteria as intraerythrocytic parasites in clinically healthy human subjects.

For light microscopic examination, blood samples from 25 healthy volunteers were drawn in a Vacutainer tube with no anticoagulants (Becton Dickinson, Franklin Lakes, N.J.); blood was drawn in the conventional manner involving antisepsis of the skin and avoidance of any introduction of external microorganisms by contamination. (Since external contamination was always a possibility, particular care and precaution were exercised at all times to avoid this.

The specific procedures, as well as appropriate controls, are specified throughout the text.)

A wet mount of the serum from the clotted blood of each sample, fresh or incubated at 30°C for between 5 to 7 days, was examined by dark-field microscopy (Leitz Dialux 20) for pleomorphic bacteria.

Read more here………………..

Live Blood Analysis Training Course Week 2 – White Blood Cells

We are on week 2 now and learning about the components of the blood including white blood cells (WBC’s)..

Above is a video of a white blood cell speeded up, you can see it is chasing a bacterial form and doesn’t stop until it gets it by engulfing it.

The WBC in the video is a Neutrophil, these cells are capable of phagocytizing (ingesting) foreign cells, toxins, bacteria and viruses. Once ingested, these organisms are destroyed by release of reactive materials such as acids and enzymes.

The numbers of white blood cells are increased or decreased in various conditions.

When infection is present the WBC’s increase in number, they also become more mobile, they move back and forth between the blood, lymph and tissues.

There are several different type of WBC each with its own unique function.

Neutrophils (above in video) are capable of ingesting toxins, foreign cells, bacteria and viruses. Eosinophils deal with parasitic infections.

Basophils are chiefly responsible for allergic and antigen response.  

Lymphocytes are responsible for immunity

Monocytes defend the body against viruses and bacteria.

We provide recognition charts to be able to identify the different WBC’s easily.

The presence of different WBC’s can indicate infections, inflammation and allergic reactions.

Too many or too few WBC’s is very important in the analysis of live blood as is the state of the WBC. We are looking for healthy, active, moving  WBC’s for a strong immune system. If this is not the case we supply the information on protocols, lifestyle changes, herbs or supplements needed to boost the immune system and expect to see improvement of the WBC on subsequent analysis.

For more information on this fascinating subject please visit our website https://livebloodonline.com

Parasites as seen in dry blood analysis (Oxidative Stress Test)

We are on week 9 of the latest live blood analysis online training course and now studying dry blood analysis which is also known as the oxidative stress test.

We are now looking at dry blood under the microscope with a higher magnification.

This is where we can see signs of parasites – what we actually see is the toxins that parasites leave behind. This adds to the toxicity of the body.

Parasites in Live Blood Analysis



Large, black ballooned out bulges clearly visible in dry blood sample.


♦ Indicates the presence of parasites and the need for a parasite cleansing program.

♦ The area in the sample where they are most concentrated may indicate where the parasites are located. (This is where we look at the concentric ring map)

Read more about parasites here……………….

Read about dry blood analysis and the oxidative stress test here……………

Copyright Dr Okker R. Botha, Johannesburg, South Africa, 2009

Week 2 on the live blood analysis online training course

We are on week 2 now and learning about the components of the blood including white blood cells, red blood cells, platelets and plasma.

For example:

Red blood cells (RBCs) which are formed in the bone marrow and are stored in the body’s reservoir for the blood, namely the spleen, comprise the greatest majority of the formed elements in the blood.

The average RBC is approximately 8 micrometers (µm) in diameter and has a life span of 110 to 120 days.

Aged RBCs are removed from circulation by macrophages that ingest them in the spleen and liver. The iron is recycled from the dead RBCs and then transported back to the marrow, where it is incorporated into new RBCs.The RBCs are responsible for the transport of oxygen from the lungs to the cells and the transport of carbon dioxide from the cells to the lungs, from where it is expelled. RBCs are capable of transporting oxygen, carbon dioxide and other gases because of an iron-containing pigment within the cells called hemoglobin.

Oxygen can easily be absorbed into the RBCs, where it forms a temporary link with the iron atoms in hemoglobin. The fluid component of the blood, namely the plasma, is straw colored. The color of blood is created by the color of the RBCs, which is due to the heme group of hemoglobin. The difference in color between oxygen-rich blood (found in arteries) and oxygen-depleted blood (found in veins) is due to the state of the hemoglobin: when bound to oxygen the resulting oxyhemoglobin is scarlet, whereas the oxygen-depleted deoxyhemoglobin is darker.

This is why veins appear bluish and arteries appear pinkish in the skin.Mature RBCs in mammals do not have a nucleus and as a result, have no DNA. RBCs have nuclei during early phases of development, but lose them as they mature in order to provide more space for hemoglobin. Mammalian RBCs also lose their other cellular organelles, such as their mitochondria. As a result, they do not use any of the oxygen they transport; instead they produce the energy carrier ATP by fermentation, through the glycolysis of glucose followed by lactic acid production. Also, RBCs do not have insulin receptors in their cell membranes and therefore the uptake of glucose into the RBCs is not regulated by insulin. Because of the lack of nucleus and organelles, the RBCs cannot synthesize any RNA, and consequently they cannot divide or repair themselves.

This inability to repair itself enables us to see some history and the results of what has been occurring to the red blood giving us vital information in live blood analysis.

Copyright Dr Okker R. Botha, Johannesburg, South Africa, 2009

Live Blood Analysis Online Training Course – Week 1

We are on the first week of the latest online training course where we go through some important information on blood analysis to help our attendees understand what one can and cannot do with this technique.

We also show what we are able to access with his amazing technique.
We look at videos on how to take blood samples correctly in live and dry blood analysis (The Oxidative Stress Test).

It is really very important to use the correct technique for taking blood samples for getting reliable and accurate results.

If a practitioner does not prepare the blood samples exactly the same way every time, they will not get accurate and reliable results and sampling becomes variable.
For example, having a regular blood sugar test is not part of prevention – it will only show an imbalance once the body has failed at all its attempts to regulate blood sugar.
When you get an abnormal blood sugar reading it is at quite a late stage already and one should really have had preventative measures in place years before the abnormal result.
Live and dry blood analysis detects imbalances that may lead to disease and one can then implement measures to help minimize the likelihood of serious conditions developing in the future.

Why our live blood analysis equipment is the best there is

Not all microscopes are created equally.

Since we specialise in live blood analysis we know exactly what live blood analysis equipment specifications are needed in a live blood system.

Our high quality microscopes are manufactured in Japan to our own specifications, so there are no other systems out there that can compare.

If your microscope has not been supplied by us it’s likely that you won’t be able to detect everything that can be seen in the blood.

These 3 factors set our systems apart from other microscopes:

Strength of the light source

We use 9W LED, equivalent to 100W halogen in the HDLED system, or 50W halogen in the ST50W system. Most regular microscopes use 20W halogen, so the light is simply not bright enough for darkfield.

Type of darkfield condenser

We use an oil immersion darkfield condenser. Cheaper systems usually use a ‘dry’ condenser that provides very poor image quality.

The “on-screen” magnification

Most microscopes are set up so that the magnification on the screen is the same as through the eyepieces. In live blood analysis we prefer to have higher magnification on the screen – up to 4X more than through the eyepieces.

This means that when we use the 40X objective we have 400X through the eyepieces and 1600X on the screen.

In microscopes where the magnification through the eyepieces is equal to that on the screen the result is that the cells appear smaller on the screen.

This is not ideal because you’ll have to use the 100X objective to get close enough to the cells to assess them properly.

Usually the 100X objective is not as clear as the 40X objective and requires oil (which means you’re not able to switch back and forth between 40X and 100X, making the analysis process quite difficult.

The added benefit with the higher on-screen magnification of our system is that a massive 4000X magnification is achieved on the screen when using the 100X objective. Click here to see our microscopes.

Our range of blood analysis microscopes:

Are laboratory-grade research microscopes that feature high quality optics and a superior build quality for unparalleled durability.

We are the only blood analysis equipment supplier that offers a 2-year warranty on our systems.

Our systems have been designed to be especially suitable for all applications in the analysis of live blood.

They can be used in both brightfield and darkfield viewing of blood samples and due to the customized illumination and optical system offer a high degree of brightness and clarity.

They have been designed so that they are easy to use, so practitioners are not distracted from doing the analysis by having to constantly change settings on their microscope.

The systems are also sold with an image management system that allows practitioners to capture and save the blood images on computer.

We have four options to choose from, designed to suit every need and budget:

Click here to see our microscopes

Live Blood Analysis Training Course – Your questions answered

woman with microscope image

We receive so many questions regarding the live blood analysis training course, that we put a live webinar together to answer some of your questions. The recording is available below.

Click here for the recording: https://youtu.be/rHrzzWbCSYs

If you would like to know about the procedure of live blood analysis, the requirements for the live blood analysis training course, or if you have any other questions – please email us at: info@livebloodonlondon.com.

For info on the latest online training course please click here: https://livebloodonline.com/the-training-course/

If you are reading this after the training course has started – it is not a problem if you miss the beginning of the training course, as you will be able to catch up with the recordings of the live lessons.

We hope to see you on the training course.

Oxidative Stress/Dry Blood Analysis

Dried blood analysis (also referred to as the Oxidative Stress Test (OST) is covered in detail in the live blood online training course.

With dry blood analysis we are able to assess oxidative stress by looking at a number of anomalies.

We are also able to see the extent of oxidative damage in the live blood samples.

We look at the level of oxidative stress in the system by looking at clotting patterns in the dry blood samples.

There is a very distinct difference between the layered dried blood sample of a healthy individual and that of a chronically ill patient.

The healthy sample is a solid mat of pinkish-red dried blood with a strong, well-interconnected fibrin network.

In the presence of degeneration, toxins and other imbalances, the dried blood sample shows white areas, called polymerized protein puddles (PPPs) as well as other abnormalities that can be indicative of certain systemic conditions.

Heavy Metal Toxicity


As the blood dries on the slide, there is a natural centrifugal activity whereby the different elements in the blood spin out into rings, depending on their specific gravity.

Organs near the centre of the body create light PPPs that don’t spin out very far, whereas heavier PPPs are created by lymph and skin conditions that spin out around the outside of the layer.

The size and shape of the PPPs is also suggestive of the nature of the condition.



Our cells can be compared to an apple that turns brown when exposed to air.

Our cells can turn brown or “rust” when we breathe due to oxidative stress, this is a process caused by free radicals. Free radicals are unstable molecules that damage or “oxidize” cells throughout the body in a process called oxidative stress.

Free radicals are unstable molecules that damage or “oxidize” cells throughout the body, this is the process called oxidative stress. Over time, oxidative stress can leave our cells and tissues unable to function properly.

Some specialists claim that free radicals can have serious consequences for our health.

Free radicals are believed to contribute to disease, hardened arteries and wrinkles, they are often associated with the health problems that we experience with age.

Avoiding the causes of free radicals and adopting a lifestyle that helps fight back against them can help safeguard health by preventing oxidative stress.



As we breathe, we can’t help but make some free radicals, but many other factors in lifestyle and environment can also contribute to their production, like:

Excess calories, sugars and/or refined carbohydrates. Eating an excess of these foods cause our mitochondria to release more “exhaust,” as they burn fuel from food for energy. This creates higher levels of free radicals.

Exercising too much or too little. Exercise is an important part of any healthy lifestyle, but too much can increase oxidative stress in our bodies.

Excessive alcohol consumption. Drinking alcohol increases cytokines levels, these are inflammatory molecules that are linked to oxidative stress.

Exposure to tobacco smoke. Tobacco smoke contains toxic chemicals that lead to oxidative stress.

Exposure to air pollutants. Industry and pollution increase oxidation in our bodies.

Excessive stress. Stress increases inflammation, which further increases free radical production.

Ionizing radiation. Exposure to x-rays, radon, cell phones and air travel can contribute to oxidative stress.

Charbroiled foods. Hydrocarbons found in these foods can contribute to oxidative stress.

Fungal toxins. Environmental moulds (found in bathrooms and basements) and internal moulds and fungi related to the gut can produce toxins that increase oxidative stress.

Poor liver and gut detoxification. The liver can become inflamed and produce more free radicals when it is overwhelmed with toxins from food or the environment, especially exposure to pesticides or mercury.

Chronic infections. Hidden infections can contribute to oxidative stress.

Lack of sleep. Sleep deprivation increases oxidation.



Preventing oxidative stress can begin by avoiding the causes of free radicals above.

Prevention of free radical formation and control of oxidative stress can be improved by:

Improving breathing and oxygenation. This can help flush out toxins, free radicals and inflammatory molecules.

Eating foods that reduce oxidation. Eating foods that contain antioxidants including a diet full of many different colourful fruits and vegetables that contain antioxidants.


Using herbs. Some herbs can help reduce oxidation:

Ginkgo, Ginger, Green tea, Milk thistle, Grape seed extract, Rosemary, Turmeric.

Supplements. Supplements that can be useful in curtailing free radical production include:

N-acetylcysteine. This can boost production of glutathione which is an important antioxidant and detoxifier.

Alpha lipoic acid. This is an antioxidant that helps to improve energy production in the mitochondria.

Coenzyme Q10 (CoQ10). Another antioxidant that is important for the mitochondria.

NADH. This is important to the cycle of energy production in the mitochondria.

Live Blood Analysis (Naturopathic Microscopy) – How does it work?

By analysing a single drop of blood we are able to tell what you’ve been eating, drinking and doing!

Naturopathic Microscopy is the only technique available to natural health practitioners that will allow you to view and assess the dynamic interplay between health and disease at the cellular level.
By analysing the blood you will be able to identify various underlying imbalances, known to lead to disease, while still at an early stage – when they can still be corrected with simple natural protocols.

You will automatically improve compliance in your practice: compliance to follow the recommended program and to follow up regularly to assess the progress.

The history of live and dry blood analysis goes back more than 60 years and it is still developing today.
Several independent researchers from across the world have spent hours behind their microscopes, analysing thousands of samples and making careful observations about the differences between healthy and unhealthy blood specimens, correlating their findings with clinical symptoms and conditions.

What we have gained from all their hard work is a technique unparalleled in its accuracy and unmatched in its reliability. It has steadily grown in popularity among natural health practitioners worldwide by virtue of the insight it is able to provide on the body’s internal environment, which we refer to as the ‘terrain’.

The analysis of both live and dry blood specimens provides us with valuable insight into the body’s internal terrain, which constitutes the blood, lymphatic fluid, cerebrospinal fluid and the interstitial fluid that surrounds every single cell in the body.

Just like a fish swimming in unhealthy water will become unhealthy, so the cells in the body are poisoned by the toxins and acids in the fluid in which they are bathed.
The purpose of looking at blood under the microscope is to determine the state of the terrain – whether it is in a state of balance or imbalance.

Read more