Seeing free radical damage and toxicity with dry blood analysis and The Oxidative Stress Test (OST)

Dry blood analysis (also referred to as The Oxidative Stress Test OST) is covered in detail in the live blood online training course.

By using dry blood analysis we are able to assess oxidative stress and see many other health anomalies.

In dry blood analysis eight drops of live blood are placed on a slide and left to dry before being viewed under the microscope. When the blood is placed on the slide with a specific technique, there is a natural centrifugal activity whereby the different elements in the blood spin out into rings, depending on their specific gravity. Organs near the centre of the body create light PPP’s (white clots/areas – see below) that don’t spin out very far, whereas heavier PPP’s are created by lymph and skin conditions that spin out around the outside of the layer.

We view the sample under the microscope looking for these PPP’s (polymerized protein puddles) as well as other anomalies. PPP’s show as soft white clots, they  may appear as white dots, lakes, or rivers, and indicate free radical activity and toxicity. 

The size and shape of the PPPs is also suggestive of the nature of the condition and can indicate the extent of oxidative damage and oxidative stress in the system. 

There is a very distinct difference between the layered dried blood sample of a healthy individual and that of a chronically ill patient. The healthy sample is a solid mat of pinkish-red dried blood with a strong, well-interconnected fibrin network (left).

In the presence of degeneration, toxins and other imbalances, the dried blood sample shows many (PPPs) as well as other abnormalities that can be indicative of certain systemic conditions (right).

PPP’S POLYMERIZED PROTEIN PUDDLES. 

PPP’s are soft clots made from polymerized proteins, they  may appear as white dots, lakes, or rivers, and indicate free radical activity and toxicity. 

Small PPP’s (2 microns) disbursed throughout the sample indicate possible allergic sensitivities and are related to hypersensitivities and allergic reactions. 

Larger PPP’s (30 microns more or less) are related to physical stress, emotional stress, mental strain and physical strain. 

Large PPP’s (40+ microns) are related to degenerative conditions.

The larger the ppp’s and the greater number, the more serious the condition. 

LAYERS

In dry blood analysis the eight drops of live blood (taken with a special technique), are placed on a slide and left to dry before being viewed under the microscope. 

Each drop of blood (dot on the slide) is called a “layer”. The first and largest drop is Layer 1 and the last and smallest drop is Layer 8. 

The larger layers are related to shallow, temporary, and acute issues. The smaller layers generally relate to more deep seated, long term or chronic situations.

RINGS

This is the term referring to concentric circles in the layer (like on a target) that coincide with different parts of the body. For example, Ring 1 is in the centre of each layer, and Ring 8 is on the outside of the circle (the largest ring) of each layer. Ring 8 represents the outside of the body (skin) and each inward ring moves progressively inwards to the inside of the body.

By applying this ring to each layer of blood we are able to see where in the body the anomalies are showing.

Ring 1 (centre) represents reproductive organs, ovaries and prostate. In men, this would be related to prostate and bladder. In women, patterns here are related to anomalies in the reproductive system. 

Rings 2 and 3 show allergic hypersensitivities. 

Rings 3, 4 & 5 relate to the vital organs such as kidney, liver, gallbladder, spleen, bladder, stomach, colon, and digestion organs. Bone and joint anomalies may show in these rings.

Ring 4 relates mainly to lung or breast issues. Possible lung problems could be congestion, flu, chronic bronchitis, smoker. Breast issues could relate to breast tenderness, cysts, fibroids, lumps or problems with implants. 

Ring 5 may show anomalies relating to lymph nodes, lymph or edema. This could also be related to water retention or toxic kidneys. This ring can also show lung, breast and hormone conditions. Bone and joint issues may show in these rings. 

Ring 4, 5 and 6 relates to Thyroid and Parathyroid conditions.

Rings 6, 7 and 8 relate to the outside of the body such as skin, lymph, eyes, nose, throat, mouth, head, brain, hips, and feet. Rings 6 and 7 also relate to the connective tissues and the lymphatic and circulatory systems. Heavy metal toxicity also shows up in this ring as a dark shadow or wave at the edge of the ring. Physical or emotional stress may show up as skin problems and this ring could also suggest dental issues. 

First Direct Evidence Shows Bacteria Change Shape Inside Humans to Avoid Antibiotics

KATARZYNA MICKIEWICZ, THE CONVERSATION

Widespread antibiotic use is largely to blame for the emergence of antibiotic resistant bacteria, which is currently one of the biggest threats to global health. Not only does antibiotic resistance already cause an estimated 700,000 deaths a year, it’s also made numerous infections, including pneumonia, tuberculosis, and gonorrhoea, harder to treat.

Without knowing how to stop bacteria from developing antibiotic resistance, it’s predicted that preventable diseases could cause 10 million deaths a year by 2050.

Some of the ways that bacteria become resistant to antibiotics is through changes in the bacteria’s genome. For example, bacteria can pump the antibiotics out, or they can break the antibiotics down. They can also stop growing and divide, which makes them difficult to spot for the immune system.

However, our research has focused on another little known method that bacteria use to become antibiotic resistant. We have directly shown that bacteria can “change shape” in the human body to avoid being targeted by antibiotics – a process that requires no genetic changes for the bacteria to continue growing.

Virtually all bacteria are surrounded by a structure called the cell wall. The wall is like a thick jacket which protects against environmental stresses and prevents the cell from bursting. It gives bacteria a regular shape (for example, a rod or a sphere), and helps them divide efficiently.

Human cells don’t possess a cell wall (or “jacket”). Because of this, it’s easy for the human immune system to recognise bacteria as an enemy because its cell wall is noticeably different. And, because the cell wall exists in bacteria but not in humans, it’s an excellent target for some of our best and most commonly used antibiotics, such as penicillin. In other words, antibiotics targeting the wall can kill bacteria without harming us.

However, bacteria can occasionally survive without their cell wall. If the surrounding conditions are able to protect the bacteria from bursting, they can turn into so-called “L-forms”, which are bacteria that don’t have a cell wall. These bacteria were discovered in 1935 by Emmy Klieneberger-Nobel, who named them after the Lister Institute where she was working at the time.

In a lab, we often use sugar to create a suitably protective environment. In the human body, this change in form is typically triggered by antibiotics that target the bacteria’s cell wall, or certain immune molecules – such as lysozyme, a molecule that’s present in our tears which helps protect us from bacterial infections.

Bacteria without a cell wall often become fragile and lose their regular shape. However, they also become partially invisible to our immune system, and completely resistant to all types of antibiotics that specifically target the cell wall.

Scientists long suspected that L-form switching might contribute to recurrent infections by helping bacteria hide from the immune system and resist the antibiotics. However, it was difficult to find evidence for this theory due to the elusive nature of L-forms and lack of appropriate methods to detect them.

Watching bacteria change shape

Our study, published in Nature Communications, looked specifically at bacterial species associated with recurrent urinary tract infections (UTIs). It found that many different bacterial species – including E. coli and Enterococcus – can indeed survive as L-forms in the human body.

This is something that has never been directly proven before. We were able to detect these sneaky bacteria using fluorescent probes that recognise bacterial DNA.

We tested urine samples from elderly patients with recurrent UTIs by growing them in a petri dish high in sugars. Not only did this environment help protect bacteria from bursting, it also isolated the L-form bacteria that were present in these samples.

In a separate experiment, we were able to see the whole process take place in living zebrafish embryos in the presence of antibiotics.

Importantly, our study shows that antibiotics need to be tested in conditions more reflective of the human body. The ones that are currently used in the medical laboratory don’t provide enough protection for delicate L-forms to survive.

Before we can fully understand how important L-form switching is compared to other forms of antibiotic resistance, further research using more patients will be needed. It will also be important to investigate what role L-forms may play in other recurrent infections, such as sepsis or pulmonary infections.

Until now, research into L-forms has been a controversial field, but our hope is that these findings will motivate more research into L-forms in disease situations. Our hope is that these findings will help find a way to clear these sneaky bacteria from our body.

Combining cell wall active antibiotics with ones that would kill L-forms might be one solution of fighting antibiotic resistant infections.

Our battle with bacteria is ongoing. As we come up with new strategies to fight them, they come up with ways to fight back. Our study highlights yet another way that bacteria adapt that we’ll need to take into account in our continuing battle with infectious disease.

https://theconversation.com/antibiotic-resistance-researchers-have-directly-proven-that-bacteria-can-change-shape-inside-humans-to-avoid-antibiotics-124296

This article is republished from The Conversation under a Creative Commons license. Read the original article.

Live blood analysis questions webinar recording

We get a lot of questions about live blood analysis and the online training course.

We have recorded a short webinar of Dr Okker answering many of the most common questions asked. 

Click here to view the video https://youtu.be/Mc8Cdqfx-5k 

You will also find answers to many questions on our frequently asked questions page https://livebloodonline.com/q-a/

If you have any further questions about live & dry blood analysis & the oxidative stress test or are interested to know more about the procedure of live blood analysis & what it can do – please send us an email with your question to info@livebloodlondon.co.uk

For the latest online training course information and dates please go to https://livebloodonline.com/the-training-course/

All your questions about Live Blood Analysis answered

The September training course is coming up on Sept 24th and we are starting to get a lot of questions about live blood analysis and the online training course.

We invite you to a short live webinar to answer your questions on Tuesday Sept 27th at 7pm.

Join us live or send us your question to info@livebloodlondon.co.uk

Register for the webinar to receive a recording afterwards. 

Click here to register https://attendee.gotowebinar.com/register/7585584996978856706

If you are curious about live & dry blood analysis or the oxidative stress test or are interested to know more about the procedure of live blood analysis & what it can do, the training course, equipment or if you have any other questions – please join us on the webinar with Dr Okker Botha our course tutor on Tuesday 24th August at 7pm.

If you can’t join us, please email us your questions to info@livebloodonline.com and register for the webinar to receive a copy of the recording with your question answered (subject to time limitations of course).

The latest online training course will begin Tuesday 24th Sept, you will find more info at www.livebloodonline.com

We hope to see you on the webinar.

Elizabeth & Dr Okker

Week 10 on the live blood analysis training course

We are now looking at case studies, some of Dr Okkers own clients and some from our attendees.

We use dark field microscopy as well as bright field microscopy to perform live and dried blood analysis on a client, we then grade the anomalies seen and decide which issue to concentrate on from our analysis.

So, the blood anomalies seen in this analysis have been graded according to their severity.

A thorough questionnaire revealed that the client (female) suffered with recurrent bladder infections that started soon after getting married and had been getting progressively worse over the years.

The infections have involved the kidneys on a few occasions and have not responded to any natural products that she tried.

She follows a fairly healthy diet and lifestyle. She has tried eliminating specific foods from her diet in case they may have been to blame.

Nothing made a significant difference.

This is what showed in her live blood cell analysis:

Protein Linkage: Grade: 3/5

Chain-like formations of lemon-shaped RBCs.
Commonly associated with impaired digestion of dietary protein, from either excess protein intake or low proteolytic enzyme production by the pancreas. Especially associated with difficulty digesting dense animal protein (such as meat & chicken).
Anisocytosis:  Grade: 4/5

RBCs that vary in size, some larger and some smaller than normal.
Most often due to vitamin B12 and/or folic acid deficiency, iron deficiency, trace mineral deficiency and in some cases certain disease states and inherited forms of anemia.
Cloud Patterns:  Grade: 4/5

RBCs with indentations appearing similar to cloud drawings.
These forms indicate the presence of inflammation and are connected to an acidic pH and trace mineral deficiency. May also indicate high homocysteine levels.
Empty WBCs:  Grade: 3/5

WBCs with very sparse cytoplasm forming a thin border around the cell.
This occurs just before rupture of the WBC and is associated with a severely stressed immune system, parasitism and an unbalanced terrain.
Neutrophils – Cohesion:  Grade: 2/5

Two or more neutrophils stuck together.
This occurs due to increased chemostaxis (chemical messaging between neutrophils) and indicates possible infection, inflammation and some disease states
Neutrophils – Count Increased:  Grade: 3/5
The most common cause for an increased neutrophil count is an acute (usually bacterial) infection.Inflammation in the body can also lead to increased neutrophil levels.Excessive stress can also increase neutrophil numbers.A high neutrophil count may also be due to a number of different disease states
Neutrophils – Disrupted:  Grade: 2/5

Disrupted neutrophils is most often indicative of chronic stress on the immune system, parasitism of the WBCs and an unbalanced, acidic terrain.
May also be associated with toxicity and severe allergic reactions.
Neutrophils – Nonviable:  Grade: 3/5

One of the most important assessments to determine the state of the immune system. Neutrophils should move around actively. Round, symmetrical, immobile neutrophils are non-viable and suggest an underactive immune system. It may be caused by: mineral deficiencies, ongoing infections & antibiotics, smoking, alcohol, medication and sugar intake and digestive weakness. Stress, lack of exercise, poor sleeping patterns and yeast overgrowth can also contribute.
Bowel Pattern:  Grade: 4/5

Observed in Layers 4 – 8
A cluster of round white holes in the centre of the sample.
This indicates bowel challenges that may include bowel inflammation (colitis, enteritis), leaky gut syndrome, strictures, diverticula, irritable bowel syndrome and poor tissue integrity. The presence of bowel patterns in more than 3 layers indicates that supporting the digestive system is a high priority.
Dark Centre:  Grade: 4/5
Observed in Layers 4 – 8
The centre of the sample appears significantly darker than the rest of the sample.This is due to bowel toxicity, often coupled with digestive insufficiency and/or Candida. Possible leaky gut syndrome and poor immunity is usually a consequence of bowel toxicity. This implies that the ability of the digestive system to eliminate toxins is compromised and that the intestinal
flora is not balanced.

To see the analysts conclusion and suggested treatment along with the 2nd appointment results and conclusion – join us for the fascinating Live Blood Analysis Online Training Course beginning Tuesday September 2019 at 7pm!

Read the course outline here:
https://livebloodonline.com/the-training-course/course-content/ Find out about the right microscope for your needs:
https://livebloodonline.com/microscopes/ See some frequently asked questions:
https://livebloodonline.com/q-a/

Don’t forget that you can join the course and acquire your microscope at any time during or after the course. Email us for a complete breakdown of all the costs of setting up in GBP & USD.

Please contact us if you have any questions, would like to enrol or if you would like to know more about choosing the right microscope.

info@livebloodonline.com

Week 4 on the live blood analysis online training course

We are now on week 4 of the Live Blood Analysis Training Course where we are continuing with red blood cell anomalies.

We are looking at Poikilocytes, Target cells, Simple Membrane Protrusion/Acanthocytes, Echinocytes, Keratocytes, Schistocytes and Anisocytes (below).

We are learning how to identify the anomalies and about why we are seeing them.

We study the causes of the anomalies and then the best way of treating them via dietary changes, lifestyle and natural supplementation if necessary.

We can always refer to the manual and the training site for further information and to find pictures & videos of the anomalies.

Anisocytes are often associated with B12 deficiency and a client showing anisocytes will often suffer from fatigue.

Our tutor explains that the fatigue is due to 2 factors, read more here

How do you know that your treatments are working?

Do you sometimes feel like you’re working in the dark when treating chronic cases? Many practitioners do – including very experienced practitioners. This is because chronic cases typically take a long time to respond to treatment, and clients don’t notice an improvement in the first few months of treatment. Most successful practitioners rely on years of experience to know that their treatment is working. However, even these practitioners struggle with poor patient compliance, because if patients don’t feel better they lose faith and start looking for other alternatives.

  • How do you determine what the treatment is doing?
  • Is your treatment addressing the most important, fundamental issue in the patient’s case?
  • Have all the contributing factors and variables in the case been considered?
  • How is the client’s diet and lifestyle influencing the effectiveness of your treatment?

These are some of the questions that natural health practitioners struggle with every day. Well, those natural health practitioners who are not using live blood analysis.

Live blood analysis can clearly show if treatment is progressing in the correct direction. Changes seen in the client’s blood samples will indicate whether the case is improving, and the abnormalities seen in the client’s blood samples will highlight the most important areas to focus on. One of the most powerful additional benefits is that the client can see for themselves that their condition is improving, so they remain motivated and compliant during the whole treatment process, ensuring positive results in every case.

This is an example of a typical live and dry blood sample before and after treatment. The blood samples are clearly improving, indicating that the client’s health is improving at a cellular level:

Observe the changes as they are taking place in the cellular environment, at the most fundamental level, during treatment.

For more information on how to make this powerful technique a part of your practice, please join us for the next training course April 2nd


Just how can live blood analysis help prevent disease?

How many tests and procedures do you know of that are truly a part of preventative medicine? A regular blood sugar test? Yearly laboratory blood tests with your GP? CT or MRI scans?

For example, having a regular blood sugar test is not part of prevention. The reason for this is quite simple. High blood sugar means that the body has failed at all its attempts to regulate the blood sugar. An abnormally high blood sugar level is a process that takes a long time to develop, with many countermeasures used by the body in its attempts to manage the situation. If you were to prevent high blood sugar you would need to be able to identify imbalances many years before the blood sugar reads high on a standard sugar test.

Live blood analysis detects many imbalances that cannot be detected through conventional investigations. It is an invaluable tool in preventative medicine is it uncovers imbalances that, if left unchecked, will lead to a number of chronic diseases. Many of these imbalances are brought on by nutritional imbalances and poor dietary and lifestyle choices. It is a well-known fact that most chronic diseases are exacerbated by diet and lifestyle.

How do you motivate your clients to change their lifestyle?

With so many chronic diseases that have been proven to be caused by lifestyle, natural health practitioners spend much of their time telling their clients to change their diet and lifestyle. However, changing one’s diet might be one of the most difficult things to do. One of the greatest benefits of live blood analysis is being able to motivate clients to make the necessary changes, and to show them the positive impact of this. This visual impact is very important. It was shown in a study that people who were given the actual images of their damaged arteries after an angiogram were significantly more compliant in making changes to their diet and lifestyle than those who only saw the images once. Being able to see the impact of poor dietary and lifestyle choices and to refer back to those images has a very powerful effect on keeping people motivated.

Dry Blood Analysis – week 9 on the training course

Dry Blood Analysis – week 9 on the training course

We are continuing with the dry blood analysis anomalies this week but under a higher magnification.

There are quite a few anomalies that can be seen under higher magnification that relate to the degree of imbalance of the terrain.

Under higher magnification in the dry blood analysis is where we see signs of parasites, heavy metal toxicity, viral spiking – associated with chronic viral activity along with many other   indications of oxidation and degeneration.

Signs of liver flukes are seen in this image, this is quite common and usually seen with signs of liver stress in the live blood analysis.

Information is provided on the training course on how to identify this anomaly and the appropriate herbal protocol and lifestyle change to clear them. This could take many months but the results can be seen on further analysis.

Join us on the next training course in this fascinating subject Tue April 2nd


Are There Naturally Occurring Pleomorphic Bacteria in the Blood of Healthy Humans?

Ascits in darkfield
Ascits in darkfield

Dark-field microscopy of blood from healthy individuals revealed the existence of pleomorphic microorganisms.

These bacteria exhibited limited growth and susceptibility to antibiotics and could be detected by fluorescent in situ hybridization and flow cytometry. They were further characterized by analysis of their 16S rRNA and gyrB genes.

In our search for spirochetes involved in Alzheimer’s disease (13), we observed pleomorphic bacteria in the blood of healthy human subjects by dark-field microscopy.

This was a surprising finding since it is generally acknowledged that the bloodstream in healthy humans is a sterile environment (7) except when there is a breach in the integrity of the tissue membranes (6).

However, the concept of the occurrence of bacteria in the blood of healthy humans is now more plausible because of cultivation-independent laboratory approaches.

The main techniques employed in such studies include PCR amplification and sequencing of the16S ribosomal DNA (rDNA). These methods have revealed the presence of a wide diversity of microorganisms in the environment, and indeed within the human body (12).

In this report we present evidence based on molecular phylogenetic techniques and light and electron microscopy, as well as other conventional microbiological methods, for the existence of a population of bacteria in healthy human blood.

In view of the apparent controversial nature of our findings, it was encouraging to note the recent report of Nikkari et al. (14), who detected blood-associated bacterial rDNA sequences by using real-time PCR methods and a probe targeting conserved regions of bacterial 16S rDNA, and an earlier report by Tedeshi et al. (16) on the presence of pleomorphic bacteria as intraerythrocytic parasites in clinically healthy human subjects.

For light microscopic examination, blood samples from 25 healthy volunteers were drawn in a Vacutainer tube with no anticoagulants (Becton Dickinson, Franklin Lakes, N.J.); blood was drawn in the conventional manner involving antisepsis of the skin and avoidance of any introduction of external microorganisms by contamination. (Since external contamination was always a possibility, particular care and precaution were exercised at all times to avoid this.

The specific procedures, as well as appropriate controls, are specified throughout the text.)

A wet mount of the serum from the clotted blood of each sample, fresh or incubated at 30°C for between 5 to 7 days, was examined by dark-field microscopy (Leitz Dialux 20) for pleomorphic bacteria.

Read more here………………..